Description
For samples:This immunoassay kit allows for the in vitro semi-quantitative determination of human HBeAg concentrations in serum, plasma.
PRINCIPLE OF THE ASSAY The microtiter plate provided in this kit has been pre-coated with HBeAb. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated HBeAb and incubated. Then substrate solutions are added to each well. Only those wells that contain HBeAg and enzyme-conjugated HBeAb will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm